Fig 1: GB1 positively regulates lipid metabolism. (A) Western blot and (B) immunofluorescence staining assays revealed upregulation of the protein levels of PPARa and SIRT6 in HepG2 cells undergoing OA&PA culture after GB1 treatment. (C) Changes in mRNA levels of PPARa and SIRT6 after GB1 treatment. GB1 advanced lipid oxidization (D) and reduced the level of free fatty acids (E) in liver cells (200×). All data were presented as mean ± SEM of three independent experiments. *, p < 0.05, **, p < 0.01.